Single-Stranded DNA Synthesis Service

CRISPR/Cas9 technology is rapidly evolving in an unprecedented way in the field of gene editing, with its core components: gRNA and Cas9 endonuclease. Cas9 targets the specific sequence in the genome under gRNA guidance and cleaves the DNA duplex at the target site to form a DSB nick. This, in turn, triggers DNA repair mechanisms: non-homologous end-linking (NHEJ) or homologous directed repair (HDR). The NHEJ pathway introduces mutations at the DSB site, and in the presence of homologous sequences, the HDR pathway introduces gene knockin at the DSB site.
Studies have shown that when creating a CRISPR knockin, single-stranded DNA (ssDNA) as an HDR repair template has higher specificity and editing efficiency than dsDNA. CRISPR/Cas9 PlatformCB provides fast and affordable ssDNA synthesis services to a wide range of research workers. By performing pure enzymatic synthesis using asymmetric polymerase chain reaction (aPCR), we are able to provide ssDNA greater than 10 kb. As a leading biotechnology company in the field of genetic editing, we guarantee the high quality and purity of our product lines.